Advanced approaches for studying
osteoclasts in transgenic models
1-4 December 2008
Institute of Medical Sciences, University of Aberdeen
FEE: €150
(includes accommodation and meals)
(NB – payable on notification of acceptance
(see below))
ELIGIBILITY: Member of ECTS
Must have basic knowledge of bone histology and
osteoclast culture
This course will focus on a range of advanced
microscopical methods for study of osteoclasts
in the context of analyzing skeletal phenotypes.
Methods include confocal, transmission and scanning
electron microscopy and the use of microCT and
histomorphometry. Please note that histomorphometry
will focus on its application to mouse models
only.
The course will consist of overview lectures
and, importantly, 5 practical sessions in which
all techniques will be demonstrated/practiced.
We will discuss and practice a range of software
programmes to help analyze results obtained.
We welcome PhD students, technicians and researchers
who already have a basic knowledge of bone histology
and osteoclast culture and now want to expand
the range of techniques they apply in their studies.
REGISTRATION - DEADLINE 12TH SEPTEMBER
2008
To register please complete the registration
form and send, together with a short summary
(around 400 words) of your work with osteoclasts
and your practical expertise with any the techniques
mentioned above. Please also include experience
with image analysis programmes in your application.
Applications will be judged by the course organisers
on suitability and practical ability. The number
of participants will be restricted to 15.
Application deadline is September 12th and you
will be notified by September 17th if you application
has been successful or not.
Please send your application by email to admin@ectsoc.org
PROGRAMME
(Subject to change)
Advanced approaches for studying osteoclasts
in transgenic models
1-4 December 2008, Institute of Medical Sciences,
University of Aberdeen
Organisers: Miep Helfrich and Rob van ‘t
Hof
| Monday
1 December |
 |
| 12.00 |
|
Arrival at IMS, Lunch |
|
| 14.00 |
|
Introduction to
course Miep Helfrich (Aberdeen,
UK) and overview of relevant work of
the Bone and Musculoskeletal
Programme in Aberdeen Mike Rogers
(Aberdeen, UK) and Bone Unit in
Edinburgh Rob van ‘t Hof
(Edinburgh, UK) |
|
| 15.00 |
|
Introduction to immunostaining
and introduction to fluorescent microscopy
- Miep Helfrich (Aberdeen,
UK) |
|
| 15.40 |
|
Tea |
|
| 16.00 |
|
Introduction to Histology
and Histomorphometry - Rob van ‘t
Hof (Edinburgh, UK) |
|
| 17.00 |
|
Tour of the labs |
|
| 17.30 |
|
Depart to accommodation
at King’s College |
|
| 19.00 |
|
Dinner with all participants
at venue in City Centre |
|
| |
| Tuesday
2 December |
| Registration,
sessions, dinner and overnight accommodation |
|
| 09.30 |
|
MicroCT - Rob
van ‘t Hof (Edinburgh,
UK) |
|
| 10.30 |
|
Coffee |
|
| 11.00 |
|
Practical 1 |
|
| 13.00 |
|
Lunch |
|
| 14.00 |
|
Practical 2 |
|
| 16.00 |
|
Electron Microscopy
- Miep Helfrich (Aberdeen,
UK)
(Tea provided during lecture) |
|
| 17.00 |
|
Finish |
|
| Wednesday
3 December |
|
| 09.30 |
|
Confocal microscopy
(theory and probes for osteoclast studies)
- Fraser Coxon (Aberdeen,
UK) |
|
| 11.00 |
|
Practical 3 |
|
| 13.00 |
|
Lunch |
|
| 14.00 |
|
Practical 4 |
|
| 16.00 |
|
in vivo imaging of osteoclast
- Pierre Jurdic (Lyon,
France) |
|
| |
| Thursday
4 December |
|
| 09.00 |
|
Practical 5 |
|
| 10.30 |
|
Extra practical session
by choice |
|
| 12.00 |
|
Correlative microscopy
- Paul Verkade (Bristol,
UK) |
|
| 13.00 |
|
Lunch and evaluation
of course |
|
| 14.00 |
|
Depart |
|
Practical Sessions:
Participants will split up into groups of 2-3
and will rotate around the practical sessions
which will be as follows:
- Transmission electron microscopy (TEM) of
bone and osteoclasts
- Scanning electron microscopy (SEM) of osteoclasts
- Confocal microscopy of osteoclasts, using
a range of antibodies and probes to membrane
and intracellular structures
- microCT and reconstruction and analysis,
practical on ex-vivo machine, lecture will also
cover in vivo imaging by CT
- Histology and histomorphometry of bone (focussing
on mouse bone, correlation with microCT and
application to transgenic models)
A variety of other analysis methods will be shown
on request (participants will be asked prior to
the course to indicate what else they want to
see) for example: reflective light microscopy
to measure resorption pits and pit depth, a variety
of imaging programmes to display 3-dimensional
data from confocal and microCT and make measurements
and live cell imaging equipment.
Demonstrators for the practicals:
Miep Helfrich (senior lecturer, TEM, histology
and general microscopy)
Rob van ‘t Hof (senior lecturer, microCT
and histomorphometry)
Fraser Coxon (research fellow, confocal microscopy,
immunostaining and SEM)
Kevin Mackenzie (technologist, SEM, microCT and
image analysis)
Debbie Scott (PhD student, immunostaining for
confocal and TEM)
John Greenhorn (technician, tissue sectioning
for LM and TEM and immunogold staining) |
